Primers for identification of type and other archived specimens of Aphrodes leafhoppers (Hemiptera, Cicadellidae)

Bluemel, Joanna Katherine, King, Robert Andrew, Virant Doberlet, Meta and Symondson, William Oliver Christian ORCID: https://orcid.org/0000-0002-3343-4679 2011. Primers for identification of type and other archived specimens of Aphrodes leafhoppers (Hemiptera, Cicadellidae). Molecular Ecology Resources 11 (5) , pp. 770-774. 10.1111/j.1755-0998.2011.03008.x

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Abstract

Primers were developed for leafhoppers of the genus Aphrodes amplifying 84–244 bp fragments of the mitochondrial cytochrome oxidase subunit I gene. DNA was extracted from legs of over 100-year-old archived museum specimens, amplified and sequenced. The fragments contain sufficient variation to unequivocally identify the different species. The majority of the analysed museum specimens, including three specimens of the syntype series for the UK endemic species A. aestuarina (Edwards 1908), were found to have been assigned to the wrong species. This work clearly underlines the need to validate museum specimens using molecular methods where identity is in doubt, based on reliable standards for species discrimination.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Advanced Research Computing @ Cardiff (ARCCA) Biosciences
Subjects:	Q Science > QH Natural history
Uncontrolled Keywords:	cytochrome oxidase subunit I; molecular diagnostics; mtDNA; museum specimens; taxonomy
Publisher:	Wiley
ISSN:	1755-098X
Last Modified:	05 Nov 2022 15:28
URI:	https://orca.cardiff.ac.uk/id/eprint/23259

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