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Mass spectrometry analysis of oxidized phosphatidylcholine and phosphatidylethanolamine

O'Donnell, Valerie Bridget 2011. Mass spectrometry analysis of oxidized phosphatidylcholine and phosphatidylethanolamine. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1811 (11) , pp. 818-826. 10.1016/j.bbalip.2011.07.018

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Oxidized phospholipids (OxPLs) are rapidly becoming recognized as important mediators of cellular and immune signaling. They are generated either enzymatically or non-enzymatically and 100s of structures exist of which only a small fraction have been analyzed to date. Pleiotropic activities, including regulation of adhesion molecule expression, pro-coagulant activity and inhibition of Toll-like receptor signaling have been observed and some are detected in models of human and animal disease, including atherosclerosis and infection. More recently, the acute generation of specific oxidized phospholipids by cellular enzymes in immune cells was reported. Assays for analysis and quantification of OxPLs were first developed approx 15years ago, primarily for hydro(pero)xy-species. Many were based on monitoring a single precursor ion with/without LC separation, based on the PL headgroup. Others combined LC with monitoring precursor to product transitions, but were unable to provide information regarding position of oxidation on unsaturated sn-2 fatty acid due to sensitivity issues. More recently, LC/MS/MS methods for specific OxPLs have been reported that enable high sensitivity quantitation in biological samples. In this review, widely used methods for detecting and quantifying various classes of OxPL will be summarized, along with practical advice for their use. In particular, the focus will be on LC/MS/MS, which today is almost universally the method of choice.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Phospholipid; Phosphatidyethanolamine; Phosphatidylcholine; Lipoxygenase; Oxidation; Mass spectrometry
Publisher: Elsevier
ISSN: 1388-1981
Last Modified: 04 Jun 2017 03:37

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