Brasen, Jens Christian, Olsen, Lars Folk and Hallett, Maurice Bartlett  ORCID: https://orcid.org/0000-0001-8197-834X
2010.
Cell surface topology creates high Ca2+ signalling microdomains.
Cell Calcium
47
(4)
, pp. 339-349.
10.1016/j.ceca.2010.01.005
|
Abstract
It has long been speculated that cellular microdomains are important for many cellular processes, especially those involving Ca2+ signalling. Measurements of cytosolic Ca2+ report maximum concentrations of less than few micromolar, yet several cytosolic enzymes require concentrations of more than 20 microM Ca2+ to be activated. In this paper, we have resolved this apparent paradox by showing that the surface topology of cells represents an important and hitherto unrecognized feature for generating microdomains of high Ca2+ in cells. We show that whereas the standard modeling assumption of a smooth cell surface predicts only moderate localized effects, the more realistic "wrinkled" surface topology predicts that Ca2+ concentrations up to 80 microM can persist within the folds of membranes for significant times. This intra-wrinkle location may account for 5% of the total cell volume. Using different geometries of wrinkles, our simulations show that high Ca2+ microdomains will be generated most effectively by long narrow membrane wrinkles of similar dimensions to those found experimentally. This is a new concept which has not previously been considered, but which has ramifications as the intra-wrinkle location is also a strategic location at which Ca2+ acts as a regulator of the cortical cytoskeleton and plasma membrane expansion.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) |
| Uncontrolled Keywords: | Microdomains; Mathematical model; Calcium; Ca2+ |
| Publisher: | Elsevier |
| ISSN: | 0143-4160 |
| Last Modified: | 19 Oct 2022 10:21 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/24113 |
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