Male infertility-linked point mutation disrupts the Ca2+oscillation-inducing and PIP2hydrolysis activity of sperm PLCζ

Nomikos, Michail, Elgmati, Khalil, Theodoridou, Maria, Calver, Brian Lewis, Cumbes, Bevan ORCID: https://orcid.org/0000-0001-8529-0040, Nounesis, George, Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449 and Lai, Francis Anthony ORCID: https://orcid.org/0000-0003-2852-8547 2011. Male infertility-linked point mutation disrupts the Ca2+oscillation-inducing and PIP2hydrolysis activity of sperm PLCζ. Biochemical Journal 434 (2) , pp. 211-217. 10.1042/BJ20101772

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Abstract

A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP2 (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Subjects:	R Medicine > RC Internal medicine R Medicine > RZ Other systems of medicine
Uncontrolled Keywords:	calcium oscillation; egg activation; fertilization; infertility; phospholipase C (PLC); phospholipase Cζ (PLCζ)
Publisher:	Biochemical Society
ISSN:	0264-6021
Related URLs:	http://www.biochemj.org/bj/435/bj4350791...
Last Modified:	06 May 2023 20:22
URI:	https://orca.cardiff.ac.uk/id/eprint/25138

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