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Current approaches to measuring human islet-antigen specific T cell function in type 1 diabetes

Mannering, S. I., Wong, Florence Susan, Durinovic-Belló, I., Brooks-Worrell, B., Tree, T. I., Cilio, C. M., Schloot, N. C. and Mallone, R. 2010. Current approaches to measuring human islet-antigen specific T cell function in type 1 diabetes. Clinical and Experimental Immunology 162 (2) , pp. 197-209. 10.1111/j.1365-2249.2010.04237.x

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Type 1 diabetes (T1D) is an autoimmune disease caused by the T cellmediated destruction of the pancreatic insulin-producing beta cells. Currently there are no widely accepted and standardized assays available to analyse the function of autoreactive T cells involved in T1D. The development of such an assay would greatly aid efforts to understand the pathogenesis of T1D and is also urgently required to guide the development of antigen-based therapies intended to prevent, or cure, T1D. Here we describe some of the assays used currently to detect autoreactive T cells in human blood and review critically their strengths and weaknesses. The challenges and future prospects for the T cell assays are discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > R Medicine (General)
Uncontrolled Keywords: CFSE, ELISPOT, immunoblot, T cell assays, tetramer, type 1 diabetes
Publisher: Wiley-Blackwell
ISSN: 0009-9104
Last Modified: 04 Jun 2017 03:46

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