Molecular epidemiology of metallo-β-lactamase-producing pseudomonas aeruginosa isolates from Norway and Sweden shows import of international clones and local clonal expansion

Samuelsen, O., Toleman, Mark Alexander Howard ORCID: https://orcid.org/0000-0002-9497-0512, Sundsfjord, A., Rydberg, J., Leegaard, T. M., Walder, M., Lia, A., Ranheim, T. E., Rajendra, Y., Hermansen, N. O., Walsh, Timothy Rutland ORCID: https://orcid.org/0000-0003-4315-4096 and Giske, C. G. 2009. Molecular epidemiology of metallo-β-lactamase-producing pseudomonas aeruginosa isolates from Norway and Sweden shows import of international clones and local clonal expansion. Antimicrobial Agents and Chemotherapy 54 (1) , pp. 346-352. 10.1128/AAC.00824-09

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Abstract

Scandinavia is considered a region with a low prevalence of antimicrobial resistance. However, the number of multidrug-resistant (MDR) Gram-negative bacteria is increasing, including metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa. In this study MBL-producing P. aeruginosa isolates identified in Norway (n = 4) and Sweden (n = 9) from 1999 to 2007 were characterized. Two international clonal complexes (CC), CC111 (n = 8) and CC235 (n = 2), previously associated with MBL-producing isolates, were dominant. CC111 isolates (ST111/229; serotype O12; blaVIM-2) included clonally related isolates identified in Skåne County, Sweden (n = 6), and two isolates associated with importation from Greece and Denmark. In all CC111 isolates, blaVIM-2 was located in integron In59.2 or In59 variants. The two CC235 isolates (ST235/ST230; serotype O11; blaVIM-4) were imported from Greece and Cyprus, were possibly clonally related, and carried blaVIM-4 in two different integron structures. Three isolates imported from Ghana (ST233; serotype O6; blaVIM-2), Tunisia (ST654; serotype O11; blaVIM-2), and Thailand (ST260; serotype O6; blaIMP-14) were clonally unrelated. ST233 was part of a new CC (CC233) that included other MBL-producing isolates, while ST654 could also be part of a new CC associated with MBL producers. In the isolates imported from Ghana and Tunisia, blaVIM-2 was part of unusual integron structures lacking the 3′ conserved segment and associated with transposons. The blaVIM gene was found to be located on the chromosome in all isolates. Known risk factors for acquisition of MBL were reported for all patients except one. The findings suggest that both import of successful international clones and local clonal expansion contribute to the emergence of MBL-producing P. aeruginosa in Scandinavia.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0066-4804/ (accessed 24/02/2014)
Publisher:	American Society for Microbiology
ISSN:	0066-4804
Date of First Compliant Deposit:	30 March 2016
Last Modified:	08 May 2023 12:42
URI:	https://orca.cardiff.ac.uk/id/eprint/27264

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