Host adhesive activities and virulence of novel fimbrial proteins of porphyromonas gingivalis

Pierce, Deanne L., Nishiyama, So-ichiro, Liang, Shuang, Wang, Min, Triantafilou, Martha ORCID: https://orcid.org/0000-0002-8489-2602, Triantafilou, Kathy ORCID: https://orcid.org/0000-0002-7473-6278, Yoshimura, Fuminobu, Demuth, Donald R. and Hajishengallis, George 2009. Host adhesive activities and virulence of novel fimbrial proteins of porphyromonas gingivalis. Infection and Immunity 77 (8) , pp. 3294-3301. 10.1128/IAI.00262-09

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Abstract

The fimbriae of Porphyromonas gingivalis mediate critical roles in host colonization and evasion of innate defenses and comprise polymerized fimbrilin (FimA) associated with quantitatively minor accessory proteins (FimCDE) of unknown function. We now show that P. gingivalis fimbriae lacking FimCDE fail to interact with the CXC-chemokine receptor 4 (CXCR4), and bacteria expressing FimCDE-deficient fimbriae cannot exploit CXCR4 in vivo for promoting their persistence, as the wild-type organism does. Consistent with these loss-of-function experiments, purified FimC and FimD (but not FimE) were shown to interact with CXCR4. However, significantly stronger binding was observed when a combination of all three proteins was allowed to interact with CXCR4. In addition, FimC and FimD bound to fibronectin and type 1 collagen, whereas FimE failed to interact with these matrix proteins. These data and the fact that FimE is required for the association of FimCDE with P. gingivalis fimbriae suggest that FimE may recruit FimC and FimD into a functional complex, rather than directly binding host proteins. Consistent with this notion, FimE was shown to bind both FimC and FimD. In summary, the FimCDE components cooperate and impart critical adhesive and virulence properties to P. gingivalis fimbriae.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QR Microbiology
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0019-9567/ (accessed 24/02/2014)
Publisher:	American Society for Microbiology
ISSN:	0019-9567
Date of First Compliant Deposit:	30 March 2016
Last Modified:	05 May 2023 22:30
URI:	https://orca.cardiff.ac.uk/id/eprint/27288

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