CARS microscopy using linearly chirped ultrafast laser pulses

Rocha-Mendoza, Israel ORCID: https://orcid.org/0000-0002-2693-6304, Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 2009. CARS microscopy using linearly chirped ultrafast laser pulses. Presented at: Multiphoton microscopy in the biomedical sciences IX, San Jose, CA, USA, 25-27 January 2009. Published in: Periasamy, A. and So, P. T. C. eds. Proceedings of the Multiphoton Microscopy in the Biomedical Sciences IX conference, San Jose, USA, 25 January 2009. Proceedings of the SPIE , vol.7183 Bellingham, WA: SPIE, 71830T. 10.1117/12.809411

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Abstract

We have developed a home-built CARS microscope which exploits linearly-chirped ultrafast laser pulses. By using glass of high group-velocity dispersion, Stokes and Pump pulses of 150 fs duration Fourier-limited are equally chirped to pulse durations in the 0.5 ps-2.8 ps range. In this way we reduce the spectral width of the instantaneous frequency difference to the Fourier limit of the chirped pulse duration (spectral focussing). As a proof of principle, CARS spectroscopy with high spectral resolution is demonstrated on polystyrene beads. We also show, both theoretically and experimentally, that for chirped pulse durations shorter than or comparable to the Raman coherence time, maximum CARS signal occurs for a Pump arriving after the Stokes pulse. Furthermore, we demonstrate the applicability of our CARS microscope to biological sciences by performing CARS microspectroscopy on different live cells and fixed tissue samples.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Physics and Astronomy Biosciences
Subjects:	Q Science > QC Physics
Publisher:	SPIE
ISBN:	9780819474292
Last Modified:	06 Nov 2022 13:34
URI:	https://orca.cardiff.ac.uk/id/eprint/27346

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