Nordmann, Patrice, Poirel, Laurent, Carrer, Amelie, Toleman, Mark Alexander Howard ![]() ![]() |
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Abstract
Enterobacterial isolates expressing the carbapenemase NDM-1 are emerging worldwide. Twenty-seven NDM-1-positive isolates of worldwide origin were included in this study to identify these strains as not only pathogens but also colonizers of normal flora for infection control screening. Although susceptibility to carbapenems varied, a combined test (IMP/IMP + EDTA), the Etest MBL, and automated susceptibility testing by Vitek2 (bioMérieux) identified those NDM-1 producers as verified by PCR using specific primers. Screening for carriers of NDM-1 producers may be based on media such as the ChromID ESBL culture medium routinely used to screen for extended-spectrum β-lactamase producers, which gives excellent detection levels with low limits of detection ranging from 8 × 100 to 5 × 102 CFU/ml. The CHROMagar KPC culture medium had higher limits of detection (1 × 101 to 5 × 105 CFU/ml) and may be proposed for the follow-up of outbreaks of infections with NDM-1 producers. Colonies growing on these screening media can be verified as NDM-1 producers with molecular methods as described herein.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine Systems Immunity Research Institute (SIURI) |
Subjects: | Q Science > QR Microbiology |
Additional Information: | Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0095-1137/ (accessed 25/02/2014) |
Publisher: | American Society for Microbiology |
ISSN: | 0095-1137 |
Date of First Compliant Deposit: | 30 March 2016 |
Last Modified: | 10 May 2023 18:41 |
URI: | https://orca.cardiff.ac.uk/id/eprint/27427 |
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