Filter-well technology for advanced three-dimensional cell culture: perspectives for respiratory research

Berube, Kelly Ann ORCID: https://orcid.org/0000-0002-7471-7229, Pitt, Aldo, Hayden, Patrick, Prytherch, Zoë Cariad ORCID: https://orcid.org/0000-0003-0690-0184 and Job, Claire Alison 2010. Filter-well technology for advanced three-dimensional cell culture: perspectives for respiratory research. Alternatives to Laboratory Animals 38 (S1) , pp. 49-65.

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Abstract

Cell culture has long been a valuable tool for studying cell behaviour. Classical plastic substrates are two-dimensional, and usually promote cellular proliferation and inhibit differentiation. Understanding cell behaviour within complex multicellular tissues requires the systematic study of cells within the context of specific model microenvironments. A model system must mimic, to a certain degree, the in vivo situation, but, at the same time, can significantly reduce its complexity. There is increasing agreement that moving up to the third dimension provides a more physiologically-relevant and predictive model system. Moreover, many cellular processes (morphogenesis, organogenesis and pathogenesis) have been confirmed to occur exclusively when cells are ordered in a three-dimensional (3-D) manner. In order to achieve the desired in vivo phenotype, researchers can use microporous membranes for improved in vitro cell culture experiments. In the present review, we discuss the applications of filter-well technology for the advanced 3-D cell culture of human pulmonary cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	bronchial epithelium; cell culture; extracellular matrix; in vitro; lung; microporous membrane; toxicology
Publisher:	Fund for the Replacement of Animals in Medical Experiments
ISSN:	0261-1929
Last Modified:	06 Nov 2022 13:41
URI:	https://orca.cardiff.ac.uk/id/eprint/27662

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