Prevalence of SXT/R391-like integrative and conjugative elements carrying blaCMY-2 in Proteus mirabilis

Mata, Caterina, Navarro, Ferran, Miro, Elisenda, Walsh, Timothy Rutland ORCID: https://orcid.org/0000-0003-4315-4096, Mirelis, Beatriz and Toleman, Mark Alexander Howard ORCID: https://orcid.org/0000-0002-9497-0512 2011. Prevalence of SXT/R391-like integrative and conjugative elements carrying blaCMY-2 in Proteus mirabilis. Journal of Antimicrobial Chemotherapy 66 (10) , pp. 2266-2270. 10.1093/jac/dkr286

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Abstract

Objectives To characterize the vectors involved in the dissemination of blaCMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of blaCMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with blaCMY-2, int and prfC probes were performed. The genetic organization of blaCMY-2 was also studied. Results ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed that blaCMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of blaCMY-2 and hybridization analyses revealed that blaCMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates. Conclusions The prevalence of ICEs carrying blaCMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying blaCMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC β-lactamases and also of other β-lactamases, such as extended-spectrum β-lactamases and carbapenemases.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) R Medicine > RC Internal medicine
Uncontrolled Keywords:	Mobile genetic elements ; AmpC β-lactamases ; Enterobacteriaceae
Publisher:	Oxford University Press
ISSN:	0305-7453
Last Modified:	20 Oct 2022 08:14
URI:	https://orca.cardiff.ac.uk/id/eprint/27673

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