Claudin-11 decreases the invasiveness of bladder cancer cells

Awsare, Ninaad S., Martin, Tracey Amanda ORCID: https://orcid.org/0000-0003-2690-4908, Haynes, Mark D., Matthews, Philip N. and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2011. Claudin-11 decreases the invasiveness of bladder cancer cells. Oncology Reports 25 (6) , pp. 1503-1509. 10.3892/or.2011.1244

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Abstract

The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as carcinoma, but displayed more intense staining than malignant tissue on immunohistochemistry. Forced-expression of claudin-11 in T24/83 cells was confirmed by PCR, immunoprecipitation and by immunofluorescence, which demonstrated increased perinuclear claudin-11 staining. Forced expression of claudin-11 did not affect TUR (p=0.243), but significantly reduced invasion (p=0.001) while increasing cell matrix adhesion (p=0.001) and growth rates (p=0.001). The greater expression of claudin-11 in benign vs. malignant tissue and non-invasive vs. invasive cell lines, and its effect in reducing bladder cancer cell invasiveness suggests that claudin-11 may have a role in preventing cancer progression and may serve as a therapeutic target in reducing metastasis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1021-335X/ (accessed 20/02/2014).
Publisher:	Spandidos
ISSN:	1021-335X
Date of First Compliant Deposit:	30 March 2016
Last Modified:	10 May 2023 19:31
URI:	https://orca.cardiff.ac.uk/id/eprint/29578

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