Calcium-phosphate, DEAE-dextran co-precipitation, and electroporation to transfer genes into neuronal and glial cell lines

Castro, M. G., Rowe, J., Morrison, E., Tomasec, Peter, Murray, C. A., Shering, A. F. and Lowenstein, P. R. 1995. Calcium-phosphate, DEAE-dextran co-precipitation, and electroporation to transfer genes into neuronal and glial cell lines. Lowenstein, P. R. and Enquist, L. W., eds. Protocols for gene transfer in neuroscience. Towards gene therapy of neurological disorders, Oxford: John Wiley & Sons, pp. 9-24.

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Abstract

The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as needed

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Additional Information:	Chapter 2
Publisher:	John Wiley & Sons
ISBN:	9780471957669
Last Modified:	28 Jun 2019 02:41
URI:	https://orca.cardiff.ac.uk/id/eprint/31676

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