An ex vivo culture model for orthodontically induced root resorption

Wan Hassan, W. N., Stephenson, Pamela A., Waddington, Rachel J. ORCID: https://orcid.org/0000-0001-5878-1434 and Sloan, Alastair James ORCID: https://orcid.org/0000-0002-1791-0903 2012. An ex vivo culture model for orthodontically induced root resorption. Journal of dentistry 40 (5) , pp. 406-416. 10.1016/j.jdent.2012.02.002

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Abstract

Objectives Root resorption is a ubiquitous although undesirable sequela to orthodontic treatment. Current methods to investigate the pathophysiology have certain limitations. In pursuit to understand and develop treatment modalities for orthodontically induced root resorption, the ability to manipulate cells within their natural extracellular matrix in a three dimensional organotypic model is invaluable. The study aimed to develop a laboratory-based organotypic model to investigate the effect of orthodontic forces on the periodontium. Methods Mandibular slices of male Wistar rats were maintained in Trowel-typed cultures at 37 °C in 5% carbon dioxide in air for 7 days with test specimens subjected to compressive forces at 50 g and 100 g by stainless steel springs. Tissue architecture and cell viability were maintained under culture conditions. Results Osteoclast numbers increased significantly in both test groups whilst odontoclasts increased in the 50 g group. Immunohistochemistry demonstrated increased dentine sialoprotein expression in both test groups, suggesting changes in mineralization-related activity due to mechanical strain. Conclusion The study showed initial cellular and molecular changes of key markers that relate to root resorption in response to mechanical loading. Clinical significance Severe root resorption may occur when forces applied are heavy or transmitted over an extended period and could lead to mobility and tooth loss. This ex vivo model can be used to investigate cellular and molecular processes during orthodontic tooth movement which may advance the clinical management of root resorption.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Uncontrolled Keywords:	Root resorption ; Tissue culture ; Osteoclasts ; Odontoclasts ; Dentine sialoprotein
Publisher:	Elsevier
ISSN:	0300-5712
Last Modified:	04 Jan 2023 02:15
URI:	https://orca.cardiff.ac.uk/id/eprint/31921

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