Hernando, J., Pourrostami, T., Garrido, J. A., Williams, Oliver Aneurin  ORCID: https://orcid.org/0000-0002-7210-3004, Gruen, D. M., Kromka, A., Steinmuller, D. and Stutzmann, M.
2007.
Immobilization of horseradish peroxidase via an amino silane on oxidized ultrananocrystalline diamond.
Diamond and Related Materials
16
(1)
, pp. 138-143.
10.1016/j.diamond.2006.04.005
|
Abstract
We discuss the complete functionalization of nitrogen-doped ultrananocrystalline diamond (UNCD) films, starting from an oxidized surface. First, the presence of hydroxyl groups on oxidized nanocrystalline diamond (NCD) was confirmed by fluorescence microscopy. Next, the grafting of a linker molecule such as 3-aminopropylmethyldiethoxysilane on oxidized NCD was confirmed by fluorescence microscopy and X-ray photoelectron spectroscopy (XPS). Then the horseradish peroxidase (HRP) enzyme was immobilized on silane-modified initially oxidized UNCD. The HRP-modified UNCD was characterized by electrochemical techniques, such as faradaic cyclic voltammetry and the amperometric response to H2O2. This response to H2O2 is discussed in terms of the layer-by-layer configuration used and the electronic properties of conducting UNCD.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Physics and Astronomy |
| Subjects: | Q Science > QC Physics |
| Uncontrolled Keywords: | Diamond film; Nanocrystalline; Electrochemical; Biomaterials |
| Publisher: | Elsevier |
| ISSN: | 0925-9635 |
| Last Modified: | 21 Oct 2022 08:42 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/34158 |
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