Ager, Ann ![]() |
Abstract
The oxygen radical generating system of xanthineoxidase plus xanthine, which has been used as a model for the oxidative burst of activated granulocytes, is known to damage endothelium in vivo and in vitro. We previously observed effects (inhibited by catalase, and thus associated with the formation of H2O2) on several parameters of endothelial function, using a non-commercialpreparation of xanthineoxidase. Our present study demonstrates that xanthineoxidase from two different commercial sources has additional effects on endothelial morphology and ion flux that are substrate-independent (i.e. produced in the absence of added xanthine) and are attributable to the presence of pancreatin (a crude enzyme mixture used in the commercialpreparation of xanthineoxidase from milk). These effects are related to the tryptic activity of pancreatin and extend previous observations on the effects of neutral proteases on endothelialcells. Our results emphasise the practical point that studies on the effects of commercialxanthineoxidasepreparations on endothelialcells must take account of their trypsin-like activity as well as their capacity to generate oxygen products.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Medicine Systems Immunity Research Institute (SIURI) |
Subjects: | Q Science > QR Microbiology > QR180 Immunology |
Uncontrolled Keywords: | endothelialcells, proteases, pancreatin, xanthineoxidase |
Publisher: | Elsevier |
ISSN: | 0049-3848 |
Last Modified: | 21 Oct 2022 09:12 |
URI: | https://orca.cardiff.ac.uk/id/eprint/35577 |
Citation Data
Cited 13 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
![]() |
Edit Item |