Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Stimulation of endothelial cells by protease activity in commercial preparations of xanthine oxidase

Ager, Ann, Wenham, D. J. and Gordon, J. L. 1984. Stimulation of endothelial cells by protease activity in commercial preparations of xanthine oxidase. Thrombosis Research 35 (1) , pp. 43-52. 10.1016/0049-3848(84)90311-6

Full text not available from this repository.


The oxygen radical generating system of xanthineoxidase plus xanthine, which has been used as a model for the oxidative burst of activated granulocytes, is known to damage endothelium in vivo and in vitro. We previously observed effects (inhibited by catalase, and thus associated with the formation of H2O2) on several parameters of endothelial function, using a non-commercialpreparation of xanthineoxidase. Our present study demonstrates that xanthineoxidase from two different commercial sources has additional effects on endothelial morphology and ion flux that are substrate-independent (i.e. produced in the absence of added xanthine) and are attributable to the presence of pancreatin (a crude enzyme mixture used in the commercialpreparation of xanthineoxidase from milk). These effects are related to the tryptic activity of pancreatin and extend previous observations on the effects of neutral proteases on endothelialcells. Our results emphasise the practical point that studies on the effects of commercialxanthineoxidasepreparations on endothelialcells must take account of their trypsin-like activity as well as their capacity to generate oxygen products.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords: endothelialcells, proteases, pancreatin, xanthineoxidase
Publisher: Elsevier
ISSN: 0049-3848
Last Modified: 04 Jun 2017 04:15

Citation Data

Cited 13 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item