In vivo photoinduction of metallothionein in human skin by ultraviolet irradiation

Anstey, Alexander Vincent ORCID: https://orcid.org/0000-0002-6345-4144, Marks, Ronald, Long, Colin, Navabi, Hossein, Pearse, Anthony D., Wynford-Thomas, David and Jasani, Bharat 1996. In vivo photoinduction of metallothionein in human skin by ultraviolet irradiation. Journal of Pathology 178 (1) , pp. 84-88. 10.1002/(SICI)1096-9896(199601)178:1<84::AID-PATH430>3.0.CO;2-W

Full text not available from this repository.

Abstract

The aims of this study were to confirm and substantiate the in vivo cutaneous induction of metallothionein (MT) in human skin by UVR, which we have reported in brief previously, and to make a preliminary attempt to characterize the time course of this phenomenon. Buttock skin in 32 volunteers was irradiated with 2 MED of UVB and biopsies were taken at 24 h from matched non-irradiated and irradiated sites. In the kinetic study, skin biopsies from six volunteers were taken at 0, 2, 8, 24, and 48 h after 2 MED UVB irradiation. MT was immunolocalized in formalin-fixed, paraffin-embedded tissue with the monoclonal antibody E9 by an indirect immunoperoxidase method. Statistically significant differences between immunocytochemical scores were identified between non-irradiated (NI) and irradiated (I) skin within suprabasal keratinocytes (mean: NI=1·2, I=5·1; P=0·01), superficial dermal fibroblasts (mean: NI=2, I=43; P<0·001), mid-dermal fibroblasts (mean: NI=0, I=27; P<0·001), and deep dermal fibroblasts (mean: NI=0,1=11; P<0·001. In the kinetic study, no consistent rise in MT score with time was observed for the epidermal component. In dermal fibroblasts, however, the first statistically significant rise in immunocytochemically detectable MT was detected at 2 h and this was found to plateau beyond 8 h. These results confirm that ambient levels of UV irradiation are capable of inducing MT in human skin in vivo. Taken together with the relative rapidity of the response, this suggests a physiological photoprotective role for MT in human skin cells. The lack of a kinetic increase in epidermal MT may be due to high basal levels. Induction of MT in dermal fibroblasts may reflect the effects of a diffusible factor released from keratinocytes after UVR.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Uncontrolled Keywords:	metallothionein, human skin, keratinocytes, UV irradiation, dermal fibroblasts, p53, photoinduction
Publisher:	Wiley-Blackwell
ISSN:	1096-9896
Last Modified:	18 Mar 2023 02:07
URI:	https://orca.cardiff.ac.uk/id/eprint/36176

Citation Data

Cited 30 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item