Extracellular ATP induces spikes in cytosolic free Ca2+ but not in NADPH oxidase activity in neutrophils

Brasen, Jens Christian, Olsen, Lars Folke and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 2011. Extracellular ATP induces spikes in cytosolic free Ca2+ but not in NADPH oxidase activity in neutrophils. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1813 (8) , pp. 1446-1452. 10.1016/j.bbamcr.2011.05.002

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Abstract

In order to establish whether non-mitochondrial oxidase activity in human neutrophils is tightly related to cytosolic Ca2+ concentration, we simultaneously measured Ca2+ oscillations induced by ATP and oxidant production in single adherent neutrophils using confocal microscopy. ATP induced fast damped Ca2+ spikes with a period of 15 s and slower irregular spikes with a period greater than 50 s. Spikes in Ca2+ occurred in the absence of Ca2+ influx, but the amplitude was damped by inhibition of Ca2+ influx. Using the oxidation of hydroethidine as a cytosolic marker of oxidant production, we show that the generation of reactive oxygen species by neutrophils adherent to glass was accelerated by ATP. The step-up in NADPH oxidase activity followed the first elevation of cytosolic Ca2+ but, despite subsequent spikes in Ca2+ concentration, no oscillations in oxidase activity could be detected. ATP induced spikes in Ca2+ in a very reproducible way and we propose that the Ca2+ signal is an on-switch for oxidase activity, but the activity is apparently not directly correlated with spiking activity in cytosolic Ca2+.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	ATP; Calcium; Ca2+; Spikes; Neutrophils; NADPH oxidase
Publisher:	Elsevier
ISSN:	0167-4889
Last Modified:	21 Oct 2022 09:44
URI:	https://orca.cardiff.ac.uk/id/eprint/37503

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