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Propagation of infrared wavelengths through the corneal stroma with reference to hydration changes

Doutch, James J., Tucker, Carole, Quantock, Andrew James, Ade, Peter A. R. and Meek, Keith Michael Andrew 2006. Propagation of infrared wavelengths through the corneal stroma with reference to hydration changes. Presented at: Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, Saratov, Russia, 26-29 September 2006. Published in: Tuchin, Valery V. ed. Saratov Fall Meeting 2006: optical technologies in biophysics and medicine VIII. Proceedings of the SPIE , vol. 6535. Bellingham, WA: SPIE, 10.1117/12.740963

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We investigated the relationship between transmitted intensity in the 4000-5000cm-1(2.5-2νm) region of the near infrared spectrum along with the hydration state of the corneal stroma. During the process of oedema, as the hydration level of the cornea is increased, the tissue thickness increases in a linear fashion. The infrared transmittivity of the cornea is known to be very similar to that of an equivalent path length of water, therefore there should exist a correlation between transmitted intensity and hydration state of the cornea. We investigated this relation theoretically using a simple model and experimentally using FTIR and a simple swelling experiment on full thicknes (800νm) bovine cornea, with physiological hydration as a start point. It is found that the relationship between intensity and hydration is linear; we compare this with our theoretical observations.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Physics and Astronomy
Subjects: Q Science > QB Astronomy
Additional Information: Article no. 65350V
Publisher: SPIE
ISBN: 9780819466570
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Last Modified: 04 Jun 2017 04:34

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