In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans

Baker, Paul W., Dobson, Alan D. W. and Marchesi, Julian Roberto ORCID: https://orcid.org/0000-0002-7994-5239 2012. In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans. Mycological Progress 11 (4) , pp. 953-956. 10.1007/s11557-011-0796-4

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Abstract

Several different methods were compared to recover a significant quantity of high quality RNA from H. simulans containing RNA of marine-derived fungi. Further processing of the RNA showed that amplification products could be obtained using fungal specific primers, RNA enriched in mRNA could be recovered and an oligonucleotide-linker could be attached to cDNA. The potential applications of these methods are discussed for future studies in determining whether marine fungi could be symbiotically associated with marine sponges.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QH Natural history > QH301 Biology
Publisher:	Springer
ISSN:	1617-416X
Last Modified:	21 Oct 2022 10:56
URI:	https://orca.cardiff.ac.uk/id/eprint/41802

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