Baker, Paul W., Dobson, Alan D. W. and Marchesi, Julian Roberto ![]() |
Official URL: http://dx.doi.org/10.1007/s11557-011-0796-4
Abstract
Several different methods were compared to recover a significant quantity of high quality RNA from H. simulans containing RNA of marine-derived fungi. Further processing of the RNA showed that amplification products could be obtained using fungal specific primers, RNA enriched in mRNA could be recovered and an oligonucleotide-linker could be attached to cDNA. The potential applications of these methods are discussed for future studies in determining whether marine fungi could be symbiotically associated with marine sponges.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences Systems Immunity Research Institute (SIURI) |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Publisher: | Springer |
ISSN: | 1617-416X |
Last Modified: | 21 Oct 2022 10:56 |
URI: | https://orca.cardiff.ac.uk/id/eprint/41802 |
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