Levels of GABAa receptor subunit mRNA in rat brain following flurazepam treatment

O'Donovan, Michael Conlon ORCID: https://orcid.org/0000-0001-7073-2379, Buckland, Paul Robert and McGuffin, Peter 1992. Levels of GABAa receptor subunit mRNA in rat brain following flurazepam treatment. Journal of Psychopharmacology 6 (3) , pp. 364-369. 10.1177/026988119200600304

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Abstract

The regulation of the subunit composition of GABA(A) receptors may be a mechanism by which tolerance to the effects of benzodiazepines occurs. We have investigated this hypothesis by examining the levels of mRNA which codes for the GABA(A) β1, 2, 3 and γ2 subunits. Male Wistar rats were injected once daily with either flurazepam or vehicle, sacrificed after treatment regimes of up to 32 days and the brain RNA isolated. The levels of specific mRNAs encoding the receptor subunits were measured relative to a β-actin standard. No changes were found in the levels of these mRNAs at any time points. Our results lend no support to the hypothesis that alterations in the β or γ2 subunit composition of GABA(A) receptors is the mechanism responsible for the development of tolerance to, or dependence on, benzodiazepines

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	benzodiazepines ; tolerance ; GABAA receptor ; mRNA levels
Publisher:	Sage
ISSN:	0269-8811
Last Modified:	24 Oct 2022 10:17
URI:	https://orca.cardiff.ac.uk/id/eprint/43738

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