Platelet derived growth factor (B) mRNA expression in alveolar macrophages in vivo in human asthma

Taylor, I. K., Sorooshian, M., Wangoo, A., Haynes, A. R., Kotecha, Sailesh ORCID: https://orcid.org/0000-0003-3535-7627, Mitchell, D. M. and Shaw, R. J. 1994. Platelet derived growth factor (B) mRNA expression in alveolar macrophages in vivo in human asthma. European Respiratory Journal 7 (11) , pp. 1966-1972.

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Abstract

Collagen deposition and myofibroblast proliferation beneath the epithelial basement membrane in patients with asthma is now increasingly recognized, although the molecular pathogenesis remains obscure. We have evaluated messenger ribonucleic acid (mRNA) expression of the profibrotic cytokine, platelet-derived growth factor-beta (PDGF-beta), in alveolar macrophages obtained following fibreoptic bronchoscopy and bronchoalveolar lavage in patients with asthma. Three subject groups were studied: 1) asthmatics using regular inhaled glucocorticoid medication (ASTST, n = 9), 2) asthmatics using intermittent inhaled beta 2-agonist therapy only (ASTBR, n = 10); 3) nonasthmatic control volunteers (n = 10). Alveolar macrophage mRNA was extracted and PDGF-beta mRNA quantified by reverse-transcriptase polymerase chain reaction (PCR) (RT-PCR) and expressed as the ratio to that of a control gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). There were no significant differences in PDGF-beta mRNA expression between the groups, or between all asthmatic (n = 19) and control subjects. Furthermore, there was no correlation between alveolar macrophage PDGF-beta mRNA expression and airway spirometry, or duration of glucocorticoid usage or dose. Thus, in contrast to other fibrotic lung diseases, we found little evidence of enhanced expression of PDGF-beta mRNA in alveolar macrophages in clinically stable bronchial asthma.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Alveolar macrophages ; asthma ; bronchoalveolar lavage ;platelet-derived growth factor-β ;reverse-transcriptase polymerase chain reaction ; subepithelial fibrosis
Publisher:	European Respiratory Society
ISSN:	0903-1936
Last Modified:	24 Oct 2022 10:24
URI:	https://orca.cardiff.ac.uk/id/eprint/44180

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