A highly sensitive assay for adenosine triphosphate employing an improved firefly luciferase reagent

Simpson, W. J., Fernandez, J. L., Hammond, J. R. M., Senior, P. S., McCarthy, B. J., Jago, P. H., Sidorowicz, S., Jassim, S. A. A. and Denyer, Stephen Paul 1990. A highly sensitive assay for adenosine triphosphate employing an improved firefly luciferase reagent. Letters in Applied Microbiology 11 (4) , pp. 208-210. 10.1111/j.1472-765X.1990.tb00162.x

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Abstract

An improved firefly luciferase reagent allowed reliable detection of as little as 80 fmo1/1 adenosine triphosphate (ATP). Contamination by ATP of the deionized water diluent limited the sensitivity of the assay in some laboratories. The reagent can be used to measure ATP from less than ten bacterial cells if care is taken to eliminate ATP from equipment and reagents.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology
Publisher:	Wiley-Blackwell
ISSN:	1472-765X
Last Modified:	19 Mar 2016 23:14
URI:	https://orca.cardiff.ac.uk/id/eprint/44240

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