The use of bioluminescence to study the behaviour of Listeria monocytogenes when attached to surfaces

Walker, A. J., Holah, J. T., Denyer, Stephen Paul and Stewart, G. S. A. B. 1993. The use of bioluminescence to study the behaviour of Listeria monocytogenes when attached to surfaces. Colloids and Surfaces A: Physicochemical and Engineering Aspects 77 (3) , pp. 225-229. 10.1016/0927-7757(93)80120-4

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Abstract

A recombinant derivative of Listeria monocytogenes engineered to express the lux AB genes of Vibrio harveyi has a bioluminescent phenotype that provides a rapid monitor of microbial viability. This phenotype was employed to follow bacterial attachment on to stainless steel surfaces, to monitor the growth of microcolonies upon these surfaces and to assess the resistance of the attached cells following biocide challenge. Real-time bioluminescence measurements provided data equivalent to viable count determinations, and the organism was found to become more resistant to the biocide phenol upon attachment to stainless steel surfaces.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Biofilms; Bioluminescence; Listeria monocytogenes; Phenol
Publisher:	Elsevier
ISSN:	0927-7757
Last Modified:	19 Mar 2016 23:14
URI:	https://orca.cardiff.ac.uk/id/eprint/44256

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