Possible role of α-1-microglobulin in mediating bacterial attachment to model surfaces

Wassall, M. A., Santin, M., Peluso, G. and Denyer, Stephen Paul 1998. Possible role of α-1-microglobulin in mediating bacterial attachment to model surfaces. Journal of Biomedical Materials Research 40 (3) , pp. 365-370. 10.1002/(SICI)1097-4636(19980605)40:3<365::AID-JBM4>3.0.CO;2-D

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Abstract

Urine proteins in the molecular weight range of 9-137 kDa deposit to an equal extent from pooled human urine onto glass (12.7 +/- 1.9 micrograms/cm) and polystyrene (11.8 +/- 1.8 micrograms/cm). Selective desorption of the proteins was achieved by washing with water or water/isopropanol mixtures. Irrespective of the washing process, proteins of molecular weight greater than 90 kDa remained associated with both surfaces while water washings alone removed most low molecular weight material. A 29 kDa protein, alpha-1-microglobulin, was removed from glass by water washing but required a 30% (v/v) isopropanol wash to desorb from polystyrene, implying attachment via hydrophobic bonding. The adhesion to polystyrene surfaces of Pseudomonas aeruginosa B4, a clinical isolate from a urinary tract infection (UTI), was strongly associated with the presence of alpha-1-microglobulin, which may be acting as a mediator of bacterial adhesion.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	protein adsorption; bacterial adhesion; α-1-microglobulin; Ps. aeruginosa
Publisher:	Wiley
ISSN:	1552-4965
Last Modified:	19 Mar 2016 23:14
URI:	https://orca.cardiff.ac.uk/id/eprint/44278

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