A novel diffusion cell model for the in vitro assessment of transcutaneous breast cancer therapeutics: effect of permeants on MCF-7 cells cultured within the receptor compartment

Davison, Zoë, Nicholson, Robert I., Denyer, Stephen P. and Heard, Charles M. ORCID: https://orcid.org/0000-0001-9703-9777 2010. A novel diffusion cell model for the in vitro assessment of transcutaneous breast cancer therapeutics: effect of permeants on MCF-7 cells cultured within the receptor compartment. European Journal Of Pharmaceutics and Biopharmaceutics 75 (3) , pp. 411-417. 10.1016/j.ejpb.2010.03.019

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Abstract

A novel model is described for investigating the potential efficacy of topically delivered anti-breast cancer agents. Using all-glass Franz diffusion cells, the permeation of 4-hydroxytamoxifen, two EGFR inhibitors (PD98059 and LY294002) and eicosapentaenoic acid (EPA) were determined from a fish oil vehicle across Cyclopore track etched membrane (CTEM) alone, full-thickness porcine ear skin alone and CTEM plus full-thickness porcine ear skin. Finally, the effect of the simultaneous permeation of these compounds was determined on the breast cancer cell line, MCF-7, cultured directly into the diffusion cell receptor compartments. The CTEM was found to be not rate limiting, and all compounds permeated the skin, with a large excess of EPA. The applied combined dose reduced the growth of MCF-7 cells by 66% after 7days. The following conclusions were obtained: (1) MCF-7 breast cancer cells can be successfully cultured within glass Franz diffusion cells. (2) A composite diffusion cell/cell culture model can indicate the potential efficacy of topically delivered anti-breast cancer therapeutic agents. (3) The levels of LY294002, PD98059, 4-hydroxytamoxifen and EPA delivered across full-thickness skin have a major inhibitory effect on the growth of MCF-7 breast cancer cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Breast cancer; Topical drug delivery; 4-Hydroxytamoxifen; EGFR inhibitors; PD98059; LY294002; Eicosapentaenoic acid; Cyclopore track etched membrane; Franz diffusion cell; Skin; Composite model
Publisher:	Elsevier
Last Modified:	24 Oct 2022 10:25
URI:	https://orca.cardiff.ac.uk/id/eprint/44327

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