The isomaltulose synthesising enzyme system of Serratia plymuthica

McAllister, Marion, Kelly, C. T., Doyle, E. and Fogarty, W. M. 1990. The isomaltulose synthesising enzyme system of Serratia plymuthica. Biotechnology Letters 12 (9) , pp. 667-672. 10.1007/BF01088191

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Abstract

An intracellular enzyme was located inSerratia plymuthica which produced isomaltulose from sucrose. The enzyme was purified giving a preparation with a specific activity of 1,285. It has pH and temperature optima of 6.0 and 30°C, respectively. The enzyme was stable retaining 100% activity after 2 weeks at 30°C. It had an isoelectric point at pH 9.0, a Mr of 79,500 and the Km for sucrose was 65.3mM. The enzyme converted 40% (w/v) sucrose to isomaltulose with an efficiency of 87%.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Kluwer Academic Publishers
ISSN:	1573-6776
Last Modified:	04 Jun 2017 04:47
URI:	https://orca.cardiff.ac.uk/id/eprint/44804

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