Osteogenic potential of bone marrow stromal cells on smooth, roughened, and tricalcium phosphate-modified titanium alloy surfaces

Colombo, J. S., Carley, Albert Frederick, Fleming, G. J., Crean, S. J., Sloan, Alastair James ORCID: https://orcid.org/0000-0002-1791-0903 and Waddington, Rachel Jane ORCID: https://orcid.org/0000-0001-5878-1434 2012. Osteogenic potential of bone marrow stromal cells on smooth, roughened, and tricalcium phosphate-modified titanium alloy surfaces. Internation Journal of Oral and Maxillofacial Implants 27 (5) , pp. 1029-1042.

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Abstract

PURPOSE: This study investigated the influence of smooth, roughened, and tricalcium phosphate (TCP)-coated roughened titanium-aluminum-vanadium (Ti-6Al-4V) surfaces on the osteogenic potential of rat bone marrow stromal cells (BMSCs). METHODS: Machined smooth (MS), grit-blasted roughened (MT), and roughened surfaces coated with TCP were prepared from Ti-6Al-4V. Plastic surfaces were used as a control. Surface topography and chemical characteristics were determined. Cell attachment, morphology, proliferation, and temporal expression of mRNA and protein markers associated with bone healing were examined. RESULTS: Roughness values were 0.09 ± 0.02 Μm, 2.71 ± 0.24 Μm, and 6.08 ± 0.62 Μm for MS, MT, and TCP, respectively. Cell attachment was similar on all surfaces. The cell expansion phase occurred during days 1 to 3 on MS surfaces and days 3 to 5 on MT and TCP surfaces. The earlier onset of differentiation on MS surfaces versus MT and TCP surfaces was evidenced by: high mRNA expression peak for Runx2 at day 5 on MS (day 7 on MT and TCP); higher mRNA expression for osteopontin, osteonectin, bone sialoprotein (BSP), osteocalcin, type 1 collagen, and alkaline phosphatase over days 5 to 12 on MS compared with MT and TCP; higher levels of bone matrix proteins on MS compared with MT, with only BSP detected on TCP; cell morphology consistent with descriptions of differentiating osteoblasts apparent at day 5 on MS and absent on MT. Compared to plastic surfaces, Ti-6Al-4V appeared to suppress mRNA for interleukin 1Β, tumor necrosis factor alpha, and peroxisome proliferator-activated receptor gamma expression and upregulate osteoprotegerin. ConCLUSIONS: Cell expansion was delayed on roughened Ti-6Al-4V surfaces, impeding osteoblast differentiation and bone matrix synthesis. These results disagree with a number of published studies examining pure titanium. Ti-6Al-4V surfaces appear to assist in the resolution of proinflammatory cytokines and inhibit BMSC differentiation toward adipocytes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry Dentistry Cardiff Catalysis Institute (CCI)
Subjects:	Q Science > QD Chemistry R Medicine > RK Dentistry
Uncontrolled Keywords:	tcp; ti-6al-4v; roughen; surface; bmsc; bone; bsp; titanium; osteogenic; tricalcium; alloy; marrow stromal; stromal cell; cell; osteogenic potential;
Last Modified:	12 Jul 2023 01:09
URI:	https://orca.cardiff.ac.uk/id/eprint/44944

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