In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel: complementary electron microprobe and synchrotron microbeam X-ray analyses

Morgan, Andrew John, Mosselmans, J. F. W., Charnock, J. M., Bennett, A., Winters, Carole, O'Reilly, Michael, Fisher, Peter Charles, Andre, Jane, Turner, M., Gunning, P. and Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221 2013. In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel: complementary electron microprobe and synchrotron microbeam X-ray analyses. Environmental Science & Technology 47 (2) , pp. 1073-1081. 10.1021/es302633f

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Abstract

Understanding the relationships between accumulated metal speciation in cells and tissues of ecologically significant taxa such as earthworms will improve risk assessments. Synchrotron-based μ-focus X-ray spectroscopy was used to detect, localize, and determine ligand-speciation of Zn and Pb in thin sections of two epigeic earthworm species collected from a Pb/Zn-mine soil. The findings indicated that Zn and Pb partition predominantly as typical hard acids (i.e., strong affinities for O-donors) within liverlike chloragocytes. Moreover, Zn speciation was very similar in the chloragog and intestinal epithelia but differed subtly in the kidneylike nephridial tubules; neither Zn nor Pb was detectable in the ventral nerve cord. High resolution X-ray mapping of high pressure-frozen, ultrathin, freeze-substituted sections in a transmission electron microscope (TEM), combined with conventional TEM structural analysis, identified a new cell type packed with highly organized rough endoplasmic reticulum and containing deposits of Cd (codistributed with S); there was no evidence that these cells are major depositories of Zn or Pb. These data may be used in a systems biology approach to assist in the interpretation of metal-evoked perturbations in whole-worm transcriptome and metabolome profiles.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Earth and Environmental Sciences
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QD Chemistry
Publisher:	American Chemical Society
ISSN:	0013-936X
Last Modified:	24 Oct 2022 10:44
URI:	https://orca.cardiff.ac.uk/id/eprint/45657

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