| Deng, Wensheng, Malecova, Barbora, Oelgeschlager, Thomas and Roberts, Stefan G. E. 2009. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Molecular and Cellular Biology 29 (6) , pp. 1389-1400. 10.1128/MCB.01346-08 |
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Abstract
TFIIB recognizes DNA sequence-specific motifs that can flank the TATA elements of the promoters of protein-encoding genes. The TFIIB recognition elements (BREu and BREd) can have positive or negative effects on transcription in a promoter context-dependent manner. Here we show that the BREs direct the selective recruitment of TFIIA and NC2 to the promoter. We find that TFIIA preferentially associates with BRE-containing promoters while NC2 is recruited to promoters that lack consensus BREs. The functional relevance of the BRE-dependent recruitment of TFIIA and NC2 was determined by small interfering RNA-mediated knockdown of TFIIA and NC2, both of which elicited BRE-dependent effects on transcription. Our results confirm the established functional reciprocity of TFIIA and NC2. However, our findings show that TFIIA assembly at BRE-containing promoters results in reduced transcriptional activity, while NC2 acts as a positive factor at promoters that lack functional BREs. Taken together, our results provide a basis for the selective recruitment of TFIIA and NC2 to the promoter and give new insights into the functional relationship between core promoter elements and general transcription factor activity.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences |
| Subjects: | Q Science > QR Microbiology |
| Additional Information: | Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0270-7306/ (accessed 25/02/2014) |
| Publisher: | American Society for Microbiology |
| ISSN: | 0270-7306 |
| Date of First Compliant Deposit: | 30 March 2016 |
| Last Modified: | 06 May 2023 05:12 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/45667 |
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