SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae

Durand-Dubief, Mickaël, Will, William Ryan, Petrini, Edoardo, Theodorou, Delphine, Harris, Rachael Rebecca, Crawford, Margaret Rosemary, Paszkiewicz, Konrad, Krueger, Felix, Correra, Rosa Maria, Vetter, Anna T., Miller, J. Ross, Kent, Nicholas A. ORCID: https://orcid.org/0000-0002-4114-1307 and Varga-Weisz, Patrick 2012. SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PLoS Genetics 8 (9) , e1002974. 10.1371/journal.pgen.1002974

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Abstract

Budding yeast centromeres are sequence-defined point centromeres and are, unlike in many other organisms, not embedded in heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor conserved in humans, promotes point centromere function through the formation of correct chromatin architecture at centromeres. Our determination of the genome-wide binding and nucleosome positioning properties of Fun30 shows that this enzyme is consistently enriched over centromeres and that a majority of CENs show Fun30-dependent changes in flanking nucleosome position and/or CEN core micrococcal nuclease accessibility. Fun30 deletion leads to defects in histone variant Htz1 occupancy genome-wide, including at and around most centromeres. FUN30 genetically interacts with CSE4, coding for the centromere-specific variant of histone H3, and counteracts the detrimental effect of transcription through centromeres on chromosome segregation and suppresses transcriptional noise over centromere CEN3. Previous work has shown a requirement for fission yeast and mammalian homologs of Fun30 in heterochromatin assembly. As centromeres in budding yeast are not embedded in heterochromatin, our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH426 Genetics
Publisher:	Public Library of Science
ISSN:	1553-7404
Date of First Compliant Deposit:	30 March 2016
Last Modified:	06 May 2023 08:13
URI:	https://orca.cardiff.ac.uk/id/eprint/47564

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