Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta

Larman, Mark Graham, Saunders, Christopher Matthew, Carroll, John, Lai, Francis Anthony ORCID: https://orcid.org/0000-0003-2852-8547 and Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449 2004. Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta. Journal of cell science 117 (12) , pp. 2513-2521. 10.1242/jcs.01109

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Abstract

During the first cell cycle Ca2+ oscillations are regulated in a cell cycle-dependent manner, such that the oscillations are unique to M phase. How the Ca2+ oscillations are regulated with such cell cycle stage-dependency is unknown, despite their importance for egg activation and embryo development. We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLC) that triggers Ca2+ oscillations similar to those caused by sperm. We show that PLC-induced Ca2+ oscillations also occur exclusively during M phase. The cell cycle-dependency can be explained by PLC's localisation to the pronuclei, which depends specifically upon a nuclear localisation signal sequence. Preventing pronuclear localisation of PLC by mutation of the nuclear localisation signal, or by inhibiting pronuclear formation/import, can prolong Ca2+ oscillations or allow them to occur during interphase. These data suggest a novel mechanism for regulating a PLC through nuclear sequestration and may explain the cell cycle-dependent regulation of Ca2+ oscillations following fertilisation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Uncontrolled Keywords:	Fertilisation ; Phospholipase C ; Ca2+ oscillations ; Cell cycle
Publisher:	Company of Biologists
ISSN:	1477-9137
Last Modified:	17 Oct 2022 08:34
URI:	https://orca.cardiff.ac.uk/id/eprint/484

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