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The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation

Caporilli, Simona, Yu, Yachuan, Jiang, Jianqiao and White-Cooper, Helen ORCID: 2013. The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. PLOS Genetics 9 (6) , e1003526. 10.1371/journal.pgen.1003526

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The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this gene in tissue specific transcription. We show that many Drosophila melanogaster testis-specific mRNAs require Nxt1 for their accumulation. The transcripts that require Nxt1 also depend on a testis-specific transcription complex, tMAC. We show that loss of Nxt1 leads to reduced transcription of tMAC targets. A reporter transcript from a tMAC-dependent promoter is under-expressed in Nxt1 mutants, however the same transcript accumulates in mutants if driven by a tMAC-independent promoter. Thus, in Drosophila primary spermatocytes, the transcription factor used to activate expression of a transcript, rather than the RNA sequence itself or the core transcription machinery, determines whether this expression requires Nxt1. We additionally find that transcripts from intron-less genes are more sensitive to loss of Nxt1 function than those from intron-containing genes and propose a mechanism in which transcript processing feeds back to increase activity of a tissue specific transcription complex.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Publisher: Public Library of Science
ISSN: 1553-7390
Funders: Wellcome Trust, BBSRC
Date of First Compliant Deposit: 30 March 2016
Last Modified: 11 May 2023 17:52

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