Selectivity mechanism of the mechanosensitive channel MscS revealed by probing channel subconducting states

Cox, Charles D., Nomura, T., Ziegler, C. S., Campbell, Anthony Keith, Wann, Kenneth Taylor and Martinac, B. 2013. Selectivity mechanism of the mechanosensitive channel MscS revealed by probing channel subconducting states. Nature Communications 4 (7) , 2137. 10.1038/ncomms3137

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Abstract

The mechanosensitive channel of small conductance (MscS) has been characterized at both functional and structural levels and has an integral role in the protection of bacterial cells against hypoosmotic shock. Here we investigate the role that the cytoplasmic domain has in MscS channel function by recording wild-type and mutant MscS single-channel activity in liposome patches. We report that MscS preferentially resides in subconducting states at hyperpolarising potentials when Ca2+ and Ba2+ ions are the major permeant cations. In addition, our results indicate that charged residues proximal to the seven vestibular portals and their electrostatic interactions with permeating cations determine selectivity and regulate the conductance of MscS and potentially other channels belonging to the MscS subfamily. Furthermore, our findings suggest a role for mechanosensitive channels in bacterial calcium regulation, indicative of functions other than protection against osmolarity changes that these channels possibly fulfil in bacteria.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Publisher:	Nature Publishing Group
ISSN:	2041-1723
Last Modified:	05 Jun 2017 04:05
URI:	https://orca.cardiff.ac.uk/id/eprint/49367

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