Generation and characterisation of anti-C6 monoclonal antibodies in C6-deficient mice: the search for an anti-C6 therapy.

Clayton, Lisa Victoria Jane Eynstone 2006. Generation and characterisation of anti-C6 monoclonal antibodies in C6-deficient mice: the search for an anti-C6 therapy. PhD Thesis, Cardiff University.

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Abstract

For the second approach, monoclonal antibodies were raised against rabbit, rat, mouse and human C6. The two most interesting antibodies were raised against human C6 and inhibited complement-mediated haemolysis in a cell-based assay. Both of these antibodies were species specific, excluding the possibility of testing their therapeutic properties in animal models of complement-mediated disease. Instead, an ex vivo model of cardiopulmonary bypass was established and used to test the ability of these antibodies to block soluble C5b-9 formation. Neither antibody inhibited soluble C5b-9 formation, suggesting that they might be interfering with the insertion of C6 into the cell membrane during MAC assembly.

Item Type:	Thesis (PhD)
Status:	Unpublished
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR180 Immunology
Date of First Compliant Deposit:	30 March 2016
Last Modified:	22 Jan 2018 14:11
URI:	https://orca.cardiff.ac.uk/id/eprint/54080

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