Peptide functionalized polydiacetylene liposomes act as a gluorescent turn-on sensor for bacterial Lipopolysaccharide

Wu, Junchen, Zawistowski, Adam, Ehrmann, Michael ORCID: https://orcid.org/0000-0002-1927-260X, Yi, Tao and Schmuck, Carsten 2011. Peptide functionalized polydiacetylene liposomes act as a gluorescent turn-on sensor for bacterial Lipopolysaccharide. Journal of the American Chemical Society 133 (25) , pp. 9720-9723. 10.1021/ja204013u

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Abstract

Mixed polydiacetylene (PDA) liposomes functionalized on their surface with a fluorescent pentalysine peptide derivative and histidine in a ratio of 1:9 can identify bacterial lipopolysaccharide (LPS). Upon photopolymerization of the self-assembled liposomes the initial fluorescence of the peptide-diacetylene amphiphiles is quenched. Interaction with LPS in aqueous solution or on the surface of E. coli DH5α restores the fluorescence. This increase in fluorescence is selective for LPS relative to other negatively charged analytes including nucleotides and ctDNA. This simple turn-on fluorescent sensor allows detecting LPS even at low micromolar concentrations.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QD Chemistry
Publisher:	American Chemical Society
ISSN:	0002-7863
Last Modified:	25 Oct 2022 09:02
URI:	https://orca.cardiff.ac.uk/id/eprint/56931

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