Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets

Di Napoli, Claudia, Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Pope, Iestyn ORCID: https://orcid.org/0000-0002-4104-0389, Otto, Cees, Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 2014. Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets. Journal of Biophotonics 7 (1-2) , pp. 68-76. 10.1002/jbio.201200197

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Abstract

We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (poly-unsaturated glyceryl trilinolenate, mono-unsaturated glyceryl trioleate and saturated glyceryl tricaprylate and glyceryl tristearate) in a rapid and label-free way. A systematic comparison of Raman spectra with CARS and D-CARS spectra was used to identify D-CARS spectral signatures which distinguish the disordered poly-unsaturated lipids from the more ordered saturated ones both in the CH-stretch vibration region and in the fingerprint region, without the need for lengthy CARS multiplex acquisition and analysis. D-CARS images of the lipid droplets at few selected wavenumbers clearly resolved the lipid composition differences, and exemplify the potential of this technique for label-free chemically selective rapid imaging of cytosolic lipid droplets in living cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Physics and Astronomy Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	Multiphoton microscopy; nonlinear optics; Coherent Antistokes Raman Scattering; lipids
Publisher:	Wiley-Blackwell
ISSN:	1864-063X
Funders:	BBSRC, EPSRC
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	6 November 2012
Last Modified:	15 Sep 2023 16:58
URI:	https://orca.cardiff.ac.uk/id/eprint/56959

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