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Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets

Di Napoli, Claudia, Masia, Francesco ORCID:, Pope, Iestyn ORCID:, Otto, Cees, Langbein, Wolfgang Werner ORCID: and Borri, Paola ORCID: 2014. Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets. Journal of Biophotonics 7 (1-2) , pp. 68-76. 10.1002/jbio.201200197

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We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (poly-unsaturated glyceryl trilinolenate, mono-unsaturated glyceryl trioleate and saturated glyceryl tricaprylate and glyceryl tristearate) in a rapid and label-free way. A systematic comparison of Raman spectra with CARS and D-CARS spectra was used to identify D-CARS spectral signatures which distinguish the disordered poly-unsaturated lipids from the more ordered saturated ones both in the CH-stretch vibration region and in the fingerprint region, without the need for lengthy CARS multiplex acquisition and analysis. D-CARS images of the lipid droplets at few selected wavenumbers clearly resolved the lipid composition differences, and exemplify the potential of this technique for label-free chemically selective rapid imaging of cytosolic lipid droplets in living cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Physics and Astronomy
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Multiphoton microscopy; nonlinear optics; Coherent Antistokes Raman Scattering; lipids
Publisher: Wiley-Blackwell
ISSN: 1864-063X
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 6 November 2012
Last Modified: 15 Sep 2023 16:58

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