Coincubation of human spermatozoa with chlamydia trachomatis in vitro causes increased tyrosine phosphorylation of sperm proteins

Hosseinzadeh, S., Brewis, Ian Andrew, Pacey, A. A., Moore, H. D. M. and Eley, A. 2000. Coincubation of human spermatozoa with chlamydia trachomatis in vitro causes increased tyrosine phosphorylation of sperm proteins. Infection and Immunity 68 (9) , pp. 4872-4876. 10.1128/IAI.68.9.4872-4876.2000

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Abstract

Elementary bodies (EBs) of the obligate intracellular bacteriumChlamydia trachomatis are responsible for the first step of attachment to host cells. We have studied the effects of EBs on human sperm protein tyrosine phosphorylation, which is important to sperm function. Indirect immunofluorescence using antiphosphotyrosine antibodies showed that serovar E, but not LGV, caused increased tyrosine phosphorylation which was localized to the sperm tail region. Immunoblotting revealed that serovar E caused a marked increase in tyrosine phosphorylation of 80- and 95-kDa sperm proteins, whereas serovar LGV caused increased phosphorylation of only the 80-kDa moiety. Considering the importance of tyrosine phosphorylation for sperm capacitation and other aspects of sperm function, we conclude that EBs may affect these events.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RG Gynecology and obstetrics R Medicine > RM Therapeutics. Pharmacology
Publisher:	American Society for Microbiology
ISSN:	0019-9567
Last Modified:	04 Jun 2017 06:13
URI:	https://orca.cardiff.ac.uk/id/eprint/57420

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