Recombinant human zona pellucida glycoprotein 3 induces calcium influx and acrosome reaction in human spermatozoa

Brewis, Ian Andrew, Clayton, R., Barratt, C. L. R., Hornby, D. P. J. and Moore, H. D. M. 1996. Recombinant human zona pellucida glycoprotein 3 induces calcium influx and acrosome reaction in human spermatozoa. Molecular Human Reproduction 2 (8) , pp. 583-589. 10.1093/molehr/2.8.583

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Abstract

Recombinant human ZP3 (rhuZP3) generated by Chinese hamster ovary cells transfected with a plasmid containing human ZP3 cDNA was used to study the acrosome reaction (AR) and intracellular calcium fluxes in capacitated human spermatozoa. Conditioned medium containing rhuZP3 significantly induced the AR (P <0.005) in 59.4 ± 4.7% of spermatozoa (control = 8.5 ± 3.1%) and caused complete acrosomal loss in a further 17.2 ± 3.8% of cells (control = 3.7 ± 0.7%; mean ± SEM, n = 5). Sperm motility was not affected and acrosomal exocytosis in response to rhuZP3 was also shown to be time-dependent. Basal concentrations of sperm intracellular calcium were measured (82 ± 7 nM; mean ± SEM, n = 9). A transient increase in intracellular calcium (typically up to 400–450 nM) occurred within 1 min of rhuZP3 addition and was followed by sustained lower values of calcium (200–400 nM). These responses were dependent on the amount of rhuZP3. This is the first report of zona protein-induced changes in intracellular calcium levels in human spermatozoa. The results support the premise that ZP3 is an aganist of the human sperm AR and that rhuZP3 generated in a eukaryotic cell is effective in this respect.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RG Gynecology and obstetrics
Uncontrolled Keywords:	acrosome reaction; calcium; human; spermatozoa; ZP3
Publisher:	Oxford University Press
ISSN:	1360-9947
Last Modified:	04 Jun 2017 06:13
URI:	https://orca.cardiff.ac.uk/id/eprint/57428

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