Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)

Perez-Perez, Jose M., Serralbo, Olivier, Vanstraelen, Marleen, Gonzalez, Cristina, Criqui, Marie-Claire, Genschik, Pascal, Kondorosi, Eva and Scheres, Ben ORCID: https://orcid.org/0000-0001-5400-9578 2008. Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant Journal 53 (1) , pp. 78-89. 10.1111/j.1365-313X.2007.03312.x

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Abstract

To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QK Botany
Uncontrolled Keywords:	HOBBIT; cyclosome; root development; cell cycle; checkpoint
Publisher:	Wiley-Blackwell
ISSN:	1365-313X
Last Modified:	25 Oct 2022 09:13
URI:	https://orca.cardiff.ac.uk/id/eprint/57707

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