A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Regan, Edward M., Hallett, Andrew Jon, Wong, L.C. Caleb, Saeed, Ibrahim Q., Langdon-Jones, Emily E., Buurma, Niklaas Jan ORCID: https://orcid.org/0000-0003-0260-5057, Pope, Simon J. A. ORCID: https://orcid.org/0000-0001-9110-9711 and Estrela, Pedro 2014. A novel cobalt complex for enhancing amperometric and impedimetric DNA detection. Electrochimica Acta 128 , pp. 10-15. 10.1016/j.electacta.2013.10.028

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Abstract

In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry
Subjects:	Q Science > QD Chemistry
Uncontrolled Keywords:	DNA sensors; Intercalators; Electrochemical impedance spectroscopy; Differential pulse voltammetry
Additional Information:	Online publication date: 18 October 2013. Selection of papers from the 13th ISE Topical Meeting07-10 April 2013, Pretoria, South Africa.
Publisher:	Elsevier
ISSN:	0013-4686
Date of First Compliant Deposit:	19 May 2016
Last Modified:	17 Nov 2024 21:15
URI:	https://orca.cardiff.ac.uk/id/eprint/58141

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