Control of pH in human long-term bone marrow cultures with low-glucose medium containing zwitterion buffer lengthens the period of haemopoietic activity

Tennant, G. B., Truran, L. N., Bailey-Wood, R. and Burnett, Alan Kenneth 2000. Control of pH in human long-term bone marrow cultures with low-glucose medium containing zwitterion buffer lengthens the period of haemopoietic activity. British Journal of Haematology 109 (4) , pp. 785-787. 10.1046/j.1365-2141.2000.02002.x

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Abstract

Primary long-term bone marrow cultures grown in 40 mM HEPES-buffered McCoy's 5A medium produced granulocyte-macrophage colony-forming units (CFU-GM) for a median of 9 weeks compared with 7 weeks with CO2/bicarbonate-buffered cultures. Reducing the medium glucose concentration (from 12.5 to 2.75 mM) extended the culture longevity to 17 weeks. The median period of erythroid colony detection increased from 6 to 8 weeks. Secondary cultures (5 x 106 cord blood mononuclear cells seeded on irradiated stroma) showed statistically similar myeloid and erythroid longevity to primary cultures. Improved control of medium pH significantly improved the capacity of long-term stromal layers to maintain stem cells in vitro.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Bone Marrow Cells/cytology*,Bone Marrow Cells/drug effects,Buffers,Cell Differentiation,Cells Cultured,Colony-Forming Units Assay,Culture Media,Glucose/pharmacology,Hematopoiesis/drug effects*,Humans,Hydrogen-Ion Concentration,Time Factors Substances Buffers Culture Media Glucose
Additional Information:	Full Text Sources Blackwell Publishing EBSCO Ovid Technologies, Inc. Other Literature Sources Labome Researcher Resource - ExactAntigen/Labome Molecular Biology Databases GLUCOSE - HSDB
Publisher:	Wiley-Blackwell
ISSN:	0007-1048
Last Modified:	25 Jun 2017 04:41
URI:	https://orca.cardiff.ac.uk/id/eprint/58557

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