High FUS/TLS expression in acute myeloid leukaemia samples

Mills, K. I., Walsh, V., Gilkes, Amanda F., Sweeney, Marion Carol ORCID: https://orcid.org/0000-0003-0713-0855, Mirza, T., Woodgate, Louise, Brown, G. and Burnett, Alan Kenneth 2000. High FUS/TLS expression in acute myeloid leukaemia samples. British Journal of Haematology 108 (2) , pp. 316-321. 10.1046/j.1365-2141.2000.01883.x

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Abstract

Retinoic acid has the ability to induce differentiation in some myeloid leukaemia cell lines and has been used to induce remission in acute promyelocytic leukaemia patients. We have analysed changes in gene expression, by differential display, in HL60 cells exposed to all-trans retinoic acid (ATRA) for only 1 h. Only about 0.4% of the genes examined by this technique showed changes in expression level, and all four of the gene fragments identified were downregulated during the short 1 h exposure. Two of the fragments were novel, a third was MYC and the fourth was the FUS proto-oncogene. Northern analysis showed that FUS was downregulated within 1 h only during induced neutrophil differentiation but not at all during induced monocyte differentiation. Unlike the sensitive cell lines, ATRA-resistant cell lines did not show a downregulation of FUS over a 24 h period of exposure to ATRA. Using a semiquantitative PCR analysis, no difference in FUS levels was observed between ATRA-sensitive and -resistant cell lines. A similar analysis was carried out on primary acute myeloid leukaemia (AML), peripheral stem cell harvests (PBSC) and cord blood samples. The PBSC and cord blood samples had FUS levels that were similar or generally less than the cell lines. However, much higher levels were seen in 63% of the AML samples examined. The data presented are consistent with previous reports for a role for FUS in the promotion and maintenance of cellular proliferation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Uncontrolled Keywords:	AcuteDisease, Cell Differentiation, HL60 Cells, Heterogeneous Nuclear Ribonucleoproteins, Humans, Leukemia Myeloid / metabolism*, RNA Binding Protein FUS, Ribonucleoproteins / metabolism*, Tretinoin / pharmacology Substances Heterogeneous-Nuclear Ribonucleoproteins, RNA-Binding Protein FUS, Ribonucleoproteins, Tretinoin
Additional Information:	Publication Types Research Support, Non-U.S. Gov't Full Text Sources Blackwell Publishing EBSCO Ovid Technologies, Inc. Other Literature Sources COS Scholar Universe Molecular Biology Databases TRANS-RETINOIC ACID - HSDB
Publisher:	Wiley-Blackwell
ISSN:	0007-1048
Last Modified:	25 Oct 2022 09:32
URI:	https://orca.cardiff.ac.uk/id/eprint/58984

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