Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors

Zhu, Jieqing, Xiao, Gengfu, Xu, Yanhui, Yuan, Fang, Zheng, Congyi, Liu, Yueyong, Yan, Huimin, Cole, David ORCID: https://orcid.org/0000-0003-0028-9396, Bell, John I., Rao, Zihe, Tien, Po and Gao, George F. 2004. Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors. Biochemical and Biophysical Research Communications 319 (1) , pp. 283-288. 10.1016/j.bbrc.2004.04.141

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Abstract

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	SARS-CoV; coronavirus; spike(S) protein; fusion core; heptad repeat (HR1 and HR2); inhibition.
Publisher:	Elsevier
ISSN:	0006-291X
Last Modified:	25 Oct 2022 09:56
URI:	https://orca.cardiff.ac.uk/id/eprint/60527

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