Zhu, Jieqing, Xiao, Gengfu, Xu, Yanhui, Yuan, Fang, Zheng, Congyi, Liu, Yueyong, Yan, Huimin, Cole, David  ORCID: https://orcid.org/0000-0003-0028-9396, Bell, John I., Rao, Zihe, Tien, Po and Gao, George F.
2004.
Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors.
Biochemical and Biophysical Research Communications
319
(1)
, pp. 283-288.
10.1016/j.bbrc.2004.04.141
|
Abstract
Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Research Institutes & Centres > Systems Immunity Research Institute (SIURI) |
| Subjects: | R Medicine > R Medicine (General) |
| Uncontrolled Keywords: | SARS-CoV; coronavirus; spike(S) protein; fusion core; heptad repeat (HR1 and HR2); inhibition. |
| Publisher: | Elsevier |
| ISSN: | 0006-291X |
| Last Modified: | 25 Oct 2022 09:56 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/60527 |
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