Investigation of the value of β-glucan-specific fluorochromes for predicting the β-glucan content of the cell walls of zoopathogenic fungi

Nicholas, R. O., Williams, David Wynne ORCID: https://orcid.org/0000-0002-7351-5131 and Hunter, P. A. 1994. Investigation of the value of β-glucan-specific fluorochromes for predicting the β-glucan content of the cell walls of zoopathogenic fungi. Mycological Research 98 (6) , pp. 694-698. 10.1016/S0953-7562(09)80419-X

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Abstract

DOI: 10.1016/S0953-7562(09)80419-XGet rights and content -------------------------------------------------------------------------------- We have used the fluorescent dyes aniline blue and calcofluor white to stain the cell walls of a variety of pathogenic fungi, and compared our observations with the sensitivity of these species to inhibitors of polysaccharide biosynthesis. Calcofluor white, which interacts with 1,4-β-glucans such as chitin, chitosan and cellulose, induced fluorescence with all the fungi tested. However, aniline blue, which binds to 1,3-β-glucans, did not interact with Cryptococcus neoformans, Pityrosporum canis or Rhizopus oryzae. This apparent absence of 1,3-β-glucan correlates with the insensitivity of these three species to the antifungal echinocandin, an inhibitor of 1,3-β-glucan synthesis. Thus aniline blue and calcofluor white are useful agents for the study of the presence of β-glucans in the cell walls of pathogenic fungi.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Dentistry
Subjects:	R Medicine > RK Dentistry
ISSN:	0953-7562
Last Modified:	25 Oct 2022 10:02
URI:	https://orca.cardiff.ac.uk/id/eprint/60906

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