Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA

Williams, David Wynne ORCID: https://orcid.org/0000-0002-7351-5131, Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268, Lewis, Michael Alexander Oxenham ORCID: https://orcid.org/0000-0003-1917-0651 and Potts, Albert John Cornel 1995. Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA. Journal of Clinical Microbiology 33 (9) , pp. 2476-2479.

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Abstract

The PCR was used to amplify a targeted region of the ribosomal DNA from 84 Candida isolates. Unique product sizes were obtained for Candida guilliermondii, Candida (Torulopsis) glabrata, and Candida pseudotropicalis. Isolates of Candida albicans, Candida tropicalis, Candida stellatoidea, Candida parapsilosis, and Candida krusei could be identified following restriction digestion of the PCR products.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Last Modified:	25 Oct 2022 10:04
URI:	https://orca.cardiff.ac.uk/id/eprint/61077

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