Wernig, Marius, Tucker, Kerry Lee, Gornik, Volker, Schneiders, Angelika, Buschwald, Rachel, Wiestler, Otmar D., Barde, Yves-Alain  ORCID: https://orcid.org/0000-0002-7627-461X and Bristle, Oliver
2002.
Tau EGFP embryonic stem cells: an efficient tool for neuronal lineage selection and transplantation.
Journal of Neuroscience Research
69
(6)
, pp. 918-924.
10.1002/jnr.10395
|
Abstract
Pluripotency and the capacity for continuous self-renewal make embryonic stem (ES) cells an attractive donor source for cell-replacement strategies. A key prerequisite for a therapeutic application of ES cells is the generation of defined somatic cell populations. Here we demonstrate that a targeted insertion of the EGFP gene into the tau locus permits efficient fluorescence-activated cell sorting (FACS)-based lineage selection of ES cell-derived neurons. After in vitro differentiation of heterozygous tau EGFP ES cells into multipotent neural precursors, EGFP is selectively induced in postmitotic neurons of various neurotransmitter phenotypes. By using FACS, ES cell-derived neurons can be enriched to purities of more than 90%. Because neuron-specific EGFP fluorescence is also observed upon transplantation of ES cell-derived neural precursors, the tau EGFP mutant represents a useful tool for the in vivo analysis of grafted ES cell-derived neurons.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Biosciences |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Uncontrolled Keywords: | embryonic stem cells; neural precursors; gene targeting; fluorescence-activated cell sorting; transplantation |
| Additional Information: | Special issue: Stem cells |
| Publisher: | Wiley |
| ISSN: | 0360-4012 |
| Last Modified: | 27 Oct 2022 08:26 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/62302 |
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