Benzakour, O., Formstone, C., Rahman, S., Kanthou, C., Dennehy, U., Scully, M. F., Kakkar, V. V. and Cooper, David Neil  ORCID: https://orcid.org/0000-0002-8943-8484
1995.
Evidence for a protein S receptor(s) on human vascular smooth muscle cells. Analysis of the binding characteristics and mitogenic properties of protein S on human vascular smooth muscle cells.
Biochemical Journal -London-
308
(2)
, pp. 481-485.
|
Abstract
The presence of specific binding sites for the coagulation factor protein S (PS) on the surface of human vascular smooth muscle cells (HVSMC) is described. The binding characteristics of 125I-PS to HVSMC were studied and found to be saturable, reversible and, as described by the Hill equation, co-operative (h 1.74; Kd 0.33 nM). Autoradiographic analysis of detergent extracts of HVSMC chemically cross-linked with 125I-PS and separated by SDS/PAGE revealed radioactivity associated with two proteins with apparent molecular masses of 220 and 230 kDa respectively. The mitogenic activity of PS on HVSMC was also investigated. Protein S was shown to stimulate DNA synthesis of growth-arrested HVSMC and to support their proliferation under low-serum conditions in a sustained and dose-dependent manner.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine |
| Publisher: | Portland Press |
| ISSN: | 0264-6021 |
| Related URLs: | |
| Last Modified: | 27 Oct 2022 08:29 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/62483 |
Citation Data
Cited 27 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)